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The MTT assay is used to measure cellular metabolic activity as an indicator of cell viability, proliferation and cytotoxicity.
The MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) tetrazolium assay is a popular tool in estimating the metabolic activity of living cells. The test is based on enzymatic reduction of the lightly colored tetrazolium salt to its formazan of intense purple-blue color, which can be …
1 maj 2013 · A comparison of using the MTT and 3 [H]thymidine incorporation assays of hGM-CSF-treated TF-1 cells. A blank absorbance value of 0.065 (from wells without cells but treated with MTT) was subtracted from all absorbance values.
Sigma-Aldrich. CT01. MTT Cell Growth Assay Kit. Quick View. Sigma-Aldrich. X4626. XTT sodium salt. Quick View. Roche. ... For the MTT viability assay, we dissolve Product No. M5655 at a concentration of 5 mg/ml in RPMI-1640 without phenol red. ... ★★★★★ No rating value Be the first to write a review . This action will open a modal dialog.
24 mar 2011 · The MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay is based on the conversion of MTT into formazan crystals by living cells, which determines mitochondrial activity.
The MTT assay is as sensitive as the radioactive method, but shows a significantly lower background, especially after long-term incubation. • The MTT assay can also be used to study cell activation.
Use of a tetrazolium dye (MTT) as an indirect measure of cell number was first reported in the early 1980s (1). The NCI evaluated MTT dye reduction as a possible endpoint in a rapid screening assay (2), and this stimulated interest in the wider scientific community.
