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Always review sections using the basic hematoxylin and eosin (H&E) stain before proceeding to perform an immunohistochemical assay in order to check out the morphology of the tissue and to determine
1 cze 2023 · Figure 1 Histological analysis of DRG cells from NOD mice. (A) DRG section stained with H&E. Cytoplasmic vacuoles (arrow) are observed in sensory neurons of a 32 weeks old non diabetic NOD female (60X); (B) DRG section stained with H&E. Leukocyte infiltration at the DRG periphery of a 26 weeks old non diabetic NOD mouse (20X); (C) Semi-thin ...
H&E-stained sections of dorsal root ganglia (DRG) were evaluated blindly for histopathological lesions by a board-certified pathologist (A.D.M.); scoring was based on the presence and...
27 gru 2013 · PDF. A Practical Guide to the Histology of the Mouse provides a full-colour atlas of mouse histology. Mouse models of disease are used extensively in biomedical research with many hundreds of new models being generated each year.
23 sty 2023 · Single-nuclei RNA-seq analysis of mouse DRG. We integrated expression data from 37,384 nuclei from five mouse DRGs (Table 1, Supplementary Data 1).
11 lut 2016 · Here, we describe a simple, step-by-step protocol for the swift isolation of mouse DRG, which can be enzymatically dissociated to produce fully differentiated primary neuronal cultures, or processed for downstream analyses, such as immunohistochemistry or RNA profiling.
9 cze 2015 · Immerse for 5 min with agitation in 70% ethanol. Place slide (s) in appropriate buffer (For H&E, use H2O) for 1 min. H&E staining. Dip slide into Mayer's Hematoxylin and agitate for 30 sec. Rinse slide in H2O for 1 minute. depending on the intensity desired, repeat this step if necessary.
