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11 lut 2016 · Here, we describe a simple, step-by-step protocol for the swift isolation of mouse DRG, which can be enzymatically dissociated to produce fully differentiated primary neuronal cultures, or processed for downstream analyses, such as immunohistochemistry or RNA profiling.
Always review sections using the basic hematoxylin and eosin (H&E) stain before proceeding to perform an immunohistochemical assay in order to check out the morphology of the tissue and to determine
9 cze 2015 · Immerse for 5 min with agitation in 70% ethanol. Place slide (s) in appropriate buffer (For H&E, use H2O) for 1 min. H&E staining. Dip slide into Mayer's Hematoxylin and agitate for 30 sec. Rinse slide in H2O for 1 minute. depending on the intensity desired, repeat this step if necessary.
27 gru 2013 · A Practical Guide to the Histology of the Mouse provides a full-colour atlas of mouse histology. Mouse models of disease are used extensively in biomedical research with many hundreds of new models being generated each year.
The H&E stain provides a comprehensive picture of the microanatomy of organs and tissues. Hematoxylin precisely stains nuclear components, including heterochromatin and nucleoli, while eosin stains cytoplasmic components including collagen and elastic fibers , muscle fibers and red blood cells.
Mice are crucial partners in contemporary trans-lational science and may be our most valuable species for genetic modelling of mammalian dis-ease due to the genetic characterization of many inbred and recombinant inbred mice and of increas-ing numbers of genetically engineered mice (GEM).
1 cze 2023 · Figure 1 Histological analysis of DRG cells from NOD mice. (A) DRG section stained with H&E. Cytoplasmic vacuoles (arrow) are observed in sensory neurons of a 32 weeks old non diabetic NOD female (60X); (B) DRG section stained with H&E. Leukocyte infiltration at the DRG periphery of a 26 weeks old non diabetic NOD mouse (20X); (C) Semi-thin ...
