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HISTO: HISTOLOGY SECTIONS FOR VIEWING UNDER THE MICROSCOPE, using BRIGHTFIELD illumination Always review sections using the basic hematoxylin and eosin (H&E) stain before proceeding to perform an immunohistochemical assay in order to check out the morphology of the tissue and to determine
This protocol describes manual H&E staining of fixed, processed, paraffin-embedded, and sectioned mouse tissues. In H&E-stained tissues, the nucleic acids stain dark blue and the proteins stain red to pink or orange.
18 mar 2019 · The laboratory mouse is the foremost mammalian model used for studying human diseases and is closely anatomically related to humans.
The H&E stain provides a comprehensive picture of the microanatomy of organs and tissues. Hematoxylin precisely stains nuclear components, including heterochromatin and nucleoli, while eosin stains cytoplasmic components including collagen and elastic fibers , muscle fibers and red blood cells.
11 lut 2016 · Here, we describe a simple, step-by-step protocol for the swift isolation of mouse DRG, which can be enzymatically dissociated to produce fully differentiated primary neuronal cultures, or processed for downstream analyses, such as immunohistochemistry or RNA profiling.
24 gru 2013 · A Practical Guide to the Histology of the Mouse provides a full–colour atlas of mouse histology, aimed at veterinary and medical pathologists who are unfamiliar with mouse tissues and scientists who wish to evaluate their own mouse models.
Subjectively, slightly smaller step intervals (50-75 μm) may also be useful when evaluating mouse cardiac valves. Impact Statement: Step sectioning of bisected mouse hearts at 100 μm intervals generally provides for examination of all four cardiac valves within six step sections or fewer. HISTOLOGY AND DISCUSSION.
