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A cross-section of a dorsal root ganglion stained with azan to make it easier to see nerve fibers and connective tissue. Compare this slide with MH 050 Dorsal Root Ganglion (H&E). Ganglion Cells. - large, nerve. cell bodies. with large nuclei and prominent, red nucleoli.
H&E-stained sections of dorsal root ganglia (DRG) were evaluated blindly for histopathological lesions by a board-certified pathologist (A.D.M.); scoring was based on the presence and...
This protocol describes manual H&E staining of fixed, processed, paraffin-embedded, and sectioned mouse tissues. In H&E-stained tissues, the nucleic acids stain dark blue and the proteins stain red to pink or orange.
11 lut 2016 · Here, we describe a simple, step-by-step protocol for the swift isolation of mouse DRG, which can be enzymatically dissociated to produce fully differentiated primary neuronal cultures, or processed for downstream analyses, such as immunohistochemistry or RNA profiling.
7 lip 2022 · A huge range of stains is used in histology, from dyes and metals to labeled antibodies. Certain stains change the coloration of cells and tissues significantly, different from the color of the original dye complex, a phenomenon known as metachromasia.
27 gru 2013 · A Practical Guide to the Histology of the Mouse provides a full-colour atlas of mouse histology. Mouse models of disease are used extensively in biomedical research with many hundreds of new models being generated each year.
Use simply labeled cassettes, using indelible pencil, to fix thin slices of organs or rolls of intestine, in 10 volumes of fixative, for less than 24 hours, before transferring to 70% alcohol, for processing into paraffin blocks.
