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23 sty 2023 · We generated a transcriptome atlas of mouse, guinea pig, cynomolgus monkey, and human DRGs by implementing a common laboratory workflow and multiple data-integration approaches to generate...
11 lut 2016 · Here, we describe a simple, step-by-step protocol for the swift isolation of mouse DRG, which can be enzymatically dissociated to produce fully differentiated primary neuronal cultures, or processed for downstream analyses, such as immunohistochemistry or RNA profiling.
4 mar 2024 · We used this resource to reveal a remarkable degree of morphological and physiological diversity across the transcriptionally distinct DRG neuron subtypes. This mouse DRG neuron toolkit should enable analyses of the neuronal building blocks of somatosensation.
1 lis 2024 · Here we provide information to choose an appropriate AAV and detail an approach to inject AAVs into the L3 and L4 DRGs of C57Bl/6 J mice for the transduction of DRG cell bodies, the main source of somatosensory neurons in the murine sciatic nerve.
21 cze 2024 · Additionally, we opted to use mouse DRG marker genes for the cell-type nomenclature because mouse is the most commonly used mammalian model system, a majority of the current atlas is composed of cells/nuclei from mice, and Cre lines driven by these marker genes are available for most subtypes to help characterize their function in a range of ...
27 gru 2013 · As well as illustrating the normal microscopic anatomy of the mouse, the book also describes and explains the common anatomic variations, artefacts associated with tissue collection and background lesions to help the scientist to distinguish these changes from experimentally- induced lesions.
These findings are supported by an RNAseq study (Ray et al., 2018) that found channel subunit SCN9A expression levels are more abundant in human DRG compared to mouse data. The sequencing data support qRT-PCR data and show differences in expression levels between mice and human DRG.
