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11 lut 2016 · Here, we describe a simple, step-by-step protocol for the swift isolation of mouse DRG, which can be enzymatically dissociated to produce fully differentiated primary neuronal cultures, or processed for downstream analyses, such as immunohistochemistry or RNA profiling.
1 cze 2023 · Methods: Histopathological analysis by electron and optical microscopy in DRG samples, and mRNA expression analyzes by the microarray technique in DRG and blood leukocyte samples from NOD and C57BL/6 mice were performed.
Dorsal Root Ganglion (Azan) A cross-section of a dorsal root ganglion stained with azan to make it easier to see nerve fibers and connective tissue. Compare this slide with MH 050 Dorsal Root Ganglion (H&E). with large nuclei and prominent, red nucleoli.
For routine diagnosis, the use of H&E staining is by far preferred for viewing cellular & tissue structure detail. Learn about best practices, protocol & more.
23 sty 2023 · We generated a transcriptome atlas of mouse, guinea pig, cynomolgus monkey, and human DRGs by implementing a common laboratory workflow and multiple data-integration approaches to generate high...
9 cze 2015 · Immerse for 5 min with agitation in 70% ethanol. Place slide (s) in appropriate buffer (For H&E, use H2O) for 1 min. H&E staining. Dip slide into Mayer's Hematoxylin and agitate for 30 sec. Rinse slide in H2O for 1 minute. depending on the intensity desired, repeat this step if necessary.
23 lut 2024 · In this paper we propose a method for absolute quantification of H&E staining in the laboratory environment, using stain assessment slides. Stain assessment slides comprise of a biopolymer film applied as a label to standard pathology glass slides.
