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23 lut 2024 · The histopathological examination of tissue is the cornerstone of cancer diagnosis globally. It is based on the staining of tissue samples with histochemical dyes, such as haematoxylin and eosin (H&E), to highlight cellular components for visual interpretation by pathologists.
1 cze 2023 · The results showed that DRG cells from NOD (and NOD.RAG-2-/-) mice have a different gene expression pattern compared to C57BL/6 mice, indicating that DRG cells from NOD mice have a functional impairment. Microarray analyses indicate that these functional changes mainly affect pathways of inflammatory processes and immune response, as well as ...
11 lut 2016 · Here, we describe a simple, step-by-step protocol for the swift isolation of mouse DRG, which can be enzymatically dissociated to produce fully differentiated primary neuronal cultures, or processed for downstream analyses, such as immunohistochemistry or RNA profiling.
H&E-stained sections of dorsal root ganglia (DRG) were evaluated blindly for histopathological lesions by a board-certified pathologist (A.D.M.); scoring was based on the presence and...
27 gru 2013 · Complete phenotypic analysis of all of these models can benefit from histologic review of the tissues. This book is aimed at veterinary and medical pathologists who are unfamiliar with mouse tissues and scientists who wish to evaluate their own mouse models.
Always review sections using the basic hematoxylin and eosin (H&E) stain before proceeding to perform an immunohistochemical assay in order to check out the morphology of the tissue and to determine
Hematoxylin and Eosin stains are used in many areas of the histology laboratory, including frozen sections, fine needle aspirates, and paraffin fixed embedded tissues. To better understand what makes a well-stained slide, it is important to understand the components of the stain.
