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H&E-stained sections of dorsal root ganglia (DRG) were evaluated blindly for histopathological lesions by a board-certified pathologist (A.D.M.); scoring was based on the presence and...
23 lut 2024 · In this paper we propose a method for absolute quantification of H&E staining in the laboratory environment, using stain assessment slides. Stain assessment slides comprise of a biopolymer film applied as a label to standard pathology glass slides.
This protocol describes manual H&E staining of fixed, processed, paraffin-embedded, and sectioned mouse tissues. In H&E-stained tissues, the nucleic acids stain dark blue and the proteins stain red to pink or orange.
11 lut 2016 · Here, we describe a simple, step-by-step protocol for the swift isolation of mouse DRG, which can be enzymatically dissociated to produce fully differentiated primary neuronal cultures, or processed for downstream analyses, such as immunohistochemistry or RNA profiling.
23 sty 2023 · We generated a transcriptome atlas of mouse, guinea pig, cynomolgus monkey, and human DRGs by implementing a common laboratory workflow and multiple data-integration approaches to generate high...
Always review sections using the basic hematoxylin and eosin (H&E) stain before proceeding to perform an immunohistochemical assay in order to check out the morphology of the tissue and to determine
21 cze 2024 · For our LR pair analysis between human/mouse DRG neurons and human/mouse spinal cord neurons, we used spinal cord datasets published by the Levine lab (57, 58). Human and mouse spinal cord cell types were subsetted for all the dorsal horn cell types found in both species as described in Yadav et al. (58).
