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11 lut 2016 · Here, we describe a simple, step-by-step protocol for the swift isolation of mouse DRG, which can be enzymatically dissociated to produce fully differentiated primary neuronal cultures, or processed for downstream analyses, such as immunohistochemistry or RNA profiling.
23 sty 2023 · We generated a transcriptome atlas of mouse, guinea pig, cynomolgus monkey, and human DRGs by implementing a common laboratory workflow and multiple data-integration approaches to generate...
H&E-stained sections of dorsal root ganglia (DRG) were evaluated blindly for histopathological lesions by a board-certified pathologist (A.D.M.); scoring was based on the presence and...
1 lis 2024 · Here we provide information to choose an appropriate AAV and detail an approach to inject AAVs into the L3 and L4 DRGs of C57Bl/6 J mice for the transduction of DRG cell bodies, the main source of somatosensory neurons in the murine sciatic nerve.
23 lut 2024 · In this paper we propose a method for absolute quantification of H&E staining in the laboratory environment, using stain assessment slides. Stain assessment slides comprise of a biopolymer film applied as a label to standard pathology glass slides.
The goal of the Mouse Histology & Phenotyping Laboratory (MHPL) at Northwestern University is to assist primary investigators and investigators with standard and customized research-specific histology and phenotyping services for their non-clinical research models.
9 cze 2015 · Immerse for 5 min with agitation in 70% ethanol. Place slide (s) in appropriate buffer (For H&E, use H2O) for 1 min. H&E staining. Dip slide into Mayer's Hematoxylin and agitate for 30 sec. Rinse slide in H2O for 1 minute. depending on the intensity desired, repeat this step if necessary.
