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For routine diagnosis, the use of H&E staining is by far preferred for viewing cellular & tissue structure detail. Learn about best practices, protocol & more.
11 lut 2016 · Here, we describe a simple, step-by-step protocol for the swift isolation of mouse DRG, which can be enzymatically dissociated to produce fully differentiated primary neuronal cultures, or processed for downstream analyses, such as immunohistochemistry or RNA profiling.
9 cze 2015 · Place slide (s) in appropriate buffer (For H&E, use H2O) for 1 min. H&E staining. Dip slide into Mayer's Hematoxylin and agitate for 30 sec. Rinse slide in H2O for 1 minute. depending on the intensity desired, repeat this step if necessary. Dip slide in 1% eosin Y fo 10-30 sec with agitation.
27 gru 2013 · A Practical Guide to the Histology of the Mouse provides a full-colour atlas of mouse histology. Mouse models of disease are used extensively in biomedical research with many hundreds of new models being generated each year.
Always review sections using the basic hematoxylin and eosin (H&E) stain before proceeding to perform an immunohistochemical assay in order to check out the morphology of the tissue and to determine
This protocol describes manual H&E staining of fixed, processed, paraffin-embedded, and sectioned mouse tissues. In H&E-stained tissues, the nucleic acids stain dark blue and the proteins stain red to pink or orange.
7 lip 2022 · Staining is widely used in histopathology and diagnosis, as it allows for the identification of abnormalities in cell count and structure under the microscope. A huge range of stains is used in histology, from dyes and metals to labeled antibodies.
