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The hematoxylin and eosin (H&E) stain is the standard used for microscopic examination of tissues that have been fixed, processed, embedded, and sectioned. It can be performed manually or by automation.
H&E-stained sections of dorsal root ganglia (DRG) were evaluated blindly for histopathological lesions by a board-certified pathologist (A.D.M.); scoring was based on the presence and...
11 lut 2016 · Here, we describe a simple, step-by-step protocol for the swift isolation of mouse DRG, which can be enzymatically dissociated to produce fully differentiated primary neuronal cultures, or processed for downstream analyses, such as immunohistochemistry or RNA profiling.
27 gru 2013 · A Practical Guide to the Histology of the Mouse provides a full-colour atlas of mouse histology. Mouse models of disease are used extensively in biomedical research with many hundreds of new models being generated each year.
7 lip 2022 · Staining is widely used in histopathology and diagnosis, as it allows for the identification of abnormalities in cell count and structure under the microscope. A huge range of stains is used in histology, from dyes and metals to labeled antibodies.
The dorsal root ganglion (DRG) houses the cell bodies of primary sensory neurons and projects axons to both the peripheral site of injury and the dorsal horn of the spinal cord. Hence, it is conceivable that the DRG is a major site of nociceptive processing in both adaptive and maladaptive pain states.
Histology of nerve fibers and connective tissue in a dorsal root ganglion stained with azan.
