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Complete medium. The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium (ATCC 30-2003). To make the complete growth medium, add the following components to the base medium: 1.4 mL Gibco™ Insulin, human recombinant, zinc solution (4 mg/mL stock); Thermofisher catalog # 12585-014
MCF-7 cells were plated at 10,000 cells per cm2 in fibronectin-coated vessels and cultured for three passages in the Cancer Cell Line Medium XF. The cells exhibited ef-ficient proliferation as well as a typical - but slightly more compact - morphology as compared to traditional culture media (not shown).
Our Cancer Cell Line Medium XF was de- signed as a highly standardized serum-free and xeno-free formulation for the serial in vitro cultivation of established cancer cell lines. It further allows to cultivate or co-cultivate a range of different cancer cells, cancer-associated cells, and non-cancer cells such as immune or stromal cells.
MCF-7 is a widely studied breast cancer cell line, and it is commonly cultured in Dulbecco's Modified Eagle Medium (DMEM) supplemented with fetal bovine serum (FBS) and other additives. DMEM...
MCF-7/TAMR-1 is a tamoxifen-resistant cell line derived from the MCF-7/S0.5 human breast cancer cell line by long term treatment with the drug tamoxifen.
MCF-7 cells were plated at 10,000 cells/cm2 in fibronectin-coated vessels and cultured for three passages in the Cancer Cell Line Medium XF. The cells exhibited efficient proliferation as well as a typical but slightly more compact compared to traditional culture media (not shown).
The MCF-7 tumorsphere culture achieved approximately 2.5 population doublings per passage. The proliferation rate is dependent on cell line and may vary accordingly with other types of tumor cells. Fig. 1b:Serial passage of MCF-7 cells in the 3D Tumorsphere Medium XF results in significant increase of TFE from 2% in P1 to 28% in P9, respectively.
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powiązane z: mcf-7 cell line medium xf 1Leverage CellEDIT Service Expertise for Custom & Stable CRISPR-Edited MCF7 Cell Line. Leverage CellEDIT Expertise for Vector-free & Multiplex CRISPR-Engineered MCF7 Cell Line.
