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ve based on dye concentration. Progressive stains (e.g., Mayer’s hematoxylin) have a lower concentration of dye and selectively stain nuclear chromatin without s. aining cytoplasmic structures. The desired i. tensity is a function of time. If staining times are excessive, a progressive stain might act similarly.
Progressive stains (e.g., Mayer’s hematoxylin) have a lower concentration of dye and selectively stain nuclear chromatin. The desired intensity is a function of time.
The first step of the H&E-staining mechanism is a coulomb interaction of the positively charged nuclear stain (hematoxylin) with the negatively charged phosphate groups of the nucleic acids in the cell nucleus. The nuclei appear in dark blue to dark violet. The second step is the counter-staining with an anionic xanthene dye (eosin Y,
Mayer's haematoxylin is one of the types of haematoxylin normally used in haematoxylin-eosin stains. Its mode of staining is progressive, i.e. the longer it remains in the staining solution the more staining is achieved in the tissue.
This histological staining reagent is suitable for visualization of nuclei in tissue sections and cell preparations. This reagent does not contain alcohol and is suitable for use with all chromogens commonly used in immunohistochemical (IHC) applications. It may also be used for routine Hematoxylin and Eosin staining.
The Mayer’s Hematoxylin Solution (CS1) is intended to be used for counterstaining steps in chromogenic in situ hybridization (CISH) applications on formalin-fixed, paraffin-embedded specimens.
Hematoxylin and Eosin Stain For Frozen Section (H&E) PROCEDURE: 1. Rinse slides in 1X PBS for . 3 minutes. 2. Rinse in a gentle stream of . tap water. until OCT is washed off for . 3 minutes. 3. Stain in . Mayers Hematoxylin. for . 30 seconds. 4. Wash in a gentle stream of . tap water. for . 1 minute. 5. Blue nuclei in . 1X PBS. for . 20 ...
