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10 wrz 2024 · MacConkey agar (MAC) is a bacterial culture medium named after bacteriologist Alfred T. MacConkey (1861-1931). MacConkey agar is a selective and differentiating agar that only grows gram-negative bacterial species; it can further differentiate the gram-negative organisms based on their lactose metabolism.
MacConkey Broth is a liquid medium used for the selective cultivation of Gram-negative, lactose-fermenting bacilli in water, food and pharmaceutical raw materials as a presumptive test for coliform organisms.
MacConkey broth (single-strength and double-strength) was prepared as recom mended (Ministry of Health, 1956); the routine practice at each laboratory being to employ a good quality peptone, and a bile salt of satisfactory inhibitory power.
Remel MacConkey Broth is a liquid medium recommended for use in qualitative procedures for selective and differential isolation of enteric gram-negative bacilli. SUMMARY AND EXPLANATION.
MacConkey broth is employed in fermentation-tubes as a primary en-richment broth, just as is standard lactose broth in the United States and Canada. It must be noted, however, that, unlike standard lactose broth, the ingredients of which are so chosen as to favor the multiplication of the great-est possible number of varieties of coliform ...
MacConkey's broth and MacConkey's agar are not satisfactory media for the detection and enumeration of coliform organisms in foods. One of the most reliable methods uses violet red bile agar in pour plate counts.
Five media—MacConkey broth, lactose broth, Teepol broth, lauryl sulphate broth and glucose glutamic acid medium—have been compared as primary media for the isolation of coliform organisms from 1691 samples of water received for examination during a period of 18 months.
