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In this video, we're going to be talking about MacConkey Agar media, and its principle, composition, uses.
12 sty 2020 · MacConkey agar preparation | culture media microbiology | lactose fermentation | differential media | MacConkey agar a differential media | composition, pre...
This video lesson demonstrates the use of MacConkey agar for growing Gram-negative bacilli, as well as how to differentiate between bacteria that strongly fe...
10 sie 2022 · MacConkey agar is a selective and differential media used for the isolation and differentiation of non-fastidious gram-negative rods, particularly members of the family Enterobacteriaceae and the genus Pseudomonas. Composition of MacConkey Agar. Final pH 7.1 +/- 0.2 at 25 degrees C. Principle of MacConkey Agar.
In a sterile conical flask add 40 g of the MacConkey broth in 1000 ml of distilled water to prepare a single strength medium. In the other flask, add 80 g of the MacConkey broth in 1000 ml of distilled water to prepare a double strength medium.
MacConkey Agar is a solid, selective and differential culture media, specifically designed to promote the growth of gram-negative bacteria. Moreover, it enables the further distinction of Gram-negative organisms by assessing their ability to metabolize lactose :
MacConkey agar is a selective medium used for the isolation of non-fastidious Gram-negative rods, particularly members of the family Enterobacteriaceae and the genus Pseudomonas, and the differentiation of lactose fermenting from lactose non-fermenting Gram-negative bacilli.