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For routine diagnosis, the use of H&E staining is by far preferred for viewing cellular & tissue structure detail. Learn about best practices, protocol & more.
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Routine H&E staining and special stains play a critical role...
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- H&E Basics Part 4: Troubleshooting H&E
Though H&E stains are relatively simple to perform, there...
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Though H&E stains are relatively simple to perform, there are a variety of problems that can occur. Learn how to troubleshoot common H&E staining issues.
Controlling the amount of hematoxylin staining is certainly key to achieving a good H&E stain, but what about eosin? In this first troubleshooting tutorial w...
H&E Troubleshooting Table . Page 1 of 7 . Problem Cause Solution . 1. Nuclei not crisp, “smudgy” nuclei, nuclear bubbling or no distinct chromatin pattern seen. There is no variation in the nuclear chromatin patterns among cells, and there is no variation in chromatin staining within one nucleus. a) Fixation is incomplete.
15 maj 2020 · There are numerous variables that can affect the quality of your H&E stain. In this post we will cover some common problems you may run into. The National Society for Histotechnology publishes its blog twice weekly featuring histotechnology-focused topics.
Review the basics of H&E staining and what optimal staining should look like. Identify common problems encountered with staining techniques. Consider solutions to common artifacts that influence stain results.
13 gru 2023 · This staining technique gives an overview of the structure of the tissue and can be used in pathological diagnosis. This article follows on from Nicola’s introduction, but we’ll take an in-depth look at the stains, chemistry and method to enable you to perform this to high standards in your own lab.
