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1. www.thermofisher.com › us › enOverview of the Immunoprecipitation (IP) Technique

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   Immunoprecipitation (IP) is the small-scale affinity purification of antigens using a specific antibody that is immobilized to a solid support such as magnetic particles or agarose resin.

   • Cell Lysis Solutions

     NP-40 Cell Lysis Buffer: Cell Lysis Buffer: M-PER Mammalian...

   • Chromatin IP

     Learn more about how to desalt, buffer exchange,...

   • GST-tagged Proteins

     Glutathione agarose delivers high yield and high purity...

   • Co-IP

     Whereas a number of antibody validation strategies can be...

   • Biotinylation

     Biotinylation, also called biotin labeling, is most commonly...

   • Overview of Affinity Purification

     Instead, epitope tags are more often used for small-scale...

   • His-tagged Proteins

     Affinity purification of His-tagged proteins. Cell lysate...

   • Pull-Down Assays

     The pull-down assay is an in vitro method used to determine...

2. en.wikipedia.org › wiki › ImmunoprecipitationImmunoprecipitation - Wikipedia

   en.wikipedia.org › wiki › Immunoprecipitation

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   Immunoprecipitation (IP) is the technique of precipitating a protein antigen out of solution using an antibody that specifically binds to that particular protein. This process can be used to isolate and concentrate a particular protein from a sample containing many thousands of different proteins.

3. www.mblbio.com › bio › gThe principle and method of immunoprecipitation (IP) | MBL Life...

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   Immunoprecipitation (IP) is a method to isolate a specific antigen from a mixture, using the antigen-antibody interaction. Antigens isolated by IP are analyzed by SDS-PAGE or Western blotting.

4. www.thermofisher.com › us › enCharacterization of Antibody Specificity Using...

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   9 sie 2016 · By using immunoprecipitation in combination with mass spectrometry (IP-MS) it is possible to verify that an antibody interacts specifically with an intended target.

5. www.sciencedirect.com › topics › biochemistry-genetics-and-molecular-biologyImmunoprecipitation - an overview | ScienceDirect Topics

   www.sciencedirect.com › topics › biochemistry-genetics-and-molecular-biology

   Immunoprecipitation (IP) is defined as the isolation of an antigen using a specific antibody coupled (covalently or noncovalently) to a sedimentable matrix. From: Methods in Cell Biology, 2012

6. www.sciencedirect.com › topics › immunology-and-microbiologyImmunoprecipitation - an overview | ScienceDirect Topics

   www.sciencedirect.com › topics › immunology-and-microbiology

   Immunoprecipitation (IP) is used to separate proteins that are bound to a specific antibody from the rest of a sample, while co-IP is used to identify protein–protein interactions between the protein that bound to the antibody used for IP and additional proteins that are detected by immunoblotting.

7. www.azolifesciences.com › article › Immunoprecipitation-and-its-ApplicationsImmunoprecipitation and its Applications - AZoLifeSciences

   www.azolifesciences.com › article › Immunoprecipitation-and-its-Applications

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   Individual Protein Immunoprecipitation (IP): Individual Protein IP utilizes an antibody to isolate a specific protein of interest from cell lysate. The antibody binds to the protein and subsequently, the antibody-antigen complex is extracted using protein A-G-coupled agarose or magnetic beads.