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18 lut 2015 · V (D)J recombination is responsible for assembling the functional immunoglobulin and T cell receptor genes in cells of the immune system. This specialized DNA rearrangement is initiated by the RAG1 and RAG2 proteins, lymphoid-specific factors which collaborate to make double-strand breaks at specific sites that flank segments of coding sequence.
V (D)J recombination assembles immunoglobulin and T cell receptor genes during lymphocyte development through a series of carefully orchestrated DNA breakage and rejoining events. DNA cleavage requires a series of protein-DNA complexes containing the RAG1 and RAG2 proteins and recombination signals that flank the recombining gene segments.
1 sty 2024 · Various mechanisms associated with NHEJ diversify V(D)J junctions, while the lymphocyte-specific TdT factor further diversifies junctions via N-region addition. V(D)J recombination is tightly regulated during lymphocyte development. RAG proteins initiate the reaction from their location in recombination centres (RCs) in various antigen receptor ...
19 kwi 2002 · V(D)J recombination is initiated via introduction of DNA double-strand breaks (DSBs) between the V, D, and J segments and flanking RSs; subsequently, RS ends are precisely joined, while coding ends are modified via a process that involves potential nucleotide loss and potential nucleotide addition.
1 sty 2015 · During lymphocyte development, the diverse repertoire of functional antigen receptors is created by the process of V(D)J recombination, in which various segments of an antigen receptor locus are brought together by several highly coordinated DNA cleavage and repair steps.
V(D)J recombination undergoes regulation at multiple levels, including the regulation of RAG1/2 activity, subnuclear reposition, spatial conformation of chromatin, and Vκ recombination, and has some specific regulation mechanisms.
This process, termed V(D)J recombination, chooses a pair of segments, introduces double-strand breaks adjacent to each segment, deletes (or, in selected cases, inverts) the intervening DNA, and ligates the segments together (Fig. 1).
