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The mainstay in diagnosing infection with HCV is to initially screen high risk groups for antibodies to HCV (anti-HCV). The inclusion of serum to cut-off ratio (S/CO) in recent guidelines is helpful in deciding the supplemental assay to be used to confirm initially reactive screening results.
20 sie 2024 · The serum samples for HCV RNA detection are most affected by the storage temperature. If they cannot be detected in time, they should be centrifuged and transferred to a 4°C refrigerator for short-term storage, preferably no more than 5 days.
Therefore, the range of clinically observed HCV RNA concentrations in serum is rarely below the lower range of the limit of quantification of quantitative assays, and most NAT assays (quantitative or qualitative) will capture the majority of viraemic infections as well as treatment failures.
4 paź 2002 · We evaluated the stability of HCV RNA in serum and clotted blood samples stored at room temperature or at 4°C for 4 days with the aim of optimizing the standard procedures of processing and storage of samples.
To assess the stability of various sample types and storage conditions for quantitative detectability of hepatitis C virus (HCV) RNA viral loads, we studied serum and EDTA/citrate plasma samples obtained from 10 patients known to be positive for HCV RNA.
1 paź 1999 · Our data demonstrated that the stability of HCV RNA in PF serum specimens during short-term storage was a function of the storage temperature — the lower the temperature, the lower the probability of specimen failure.
To assess the stability of various sample types and storage conditions for quantitative detectability of hepatitis C virus (HCV) RNA viral loads, we studied serum and EDTA/citrate plasma...
