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Controlling the amount of hematoxylin staining is certainly key to achieving a good H&E stain, but what about eosin? In this first troubleshooting tutorial w...
Points to be careful for defective staining and it's troubleshooting.
Though H&E stains are relatively simple to perform, there are a variety of problems that can occur. Learn how to troubleshoot common H&E staining issues.
H&E Troubleshooting Table . Page 1 of 7 . Problem Cause Solution . 1. Nuclei not crisp, “smudgy” nuclei, nuclear bubbling or no distinct chromatin pattern seen. There is no variation in the nuclear chromatin patterns among cells, and there is no variation in chromatin staining within one nucleus. a) Fixation is incomplete.
H&E Staining Troubleshooting. The hematoxylin and eosin (H&E) stained tissue section is the cornerstone of anatomical pathology diagnosis. The H&E procedure stains the nucleus and cytoplasm contrasting colors to readily differentiate cellular components.
A first person view of how to manually stain slides using the H&E staining method.The protocol demonstrates use of Ehrlich's hematoxylin recipe which is desi...
Review the basics of H&E staining and what optimal staining should look like. Identify common problems encountered with staining techniques. Consider solutions to common artifacts that influence stain results.
