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Controlling the amount of hematoxylin staining is certainly key to achieving a good H&E stain, but what about eosin? In this first troubleshooting tutorial w...
Points to be careful for defective staining and it's troubleshooting.
Though H&E stains are relatively simple to perform, there are a variety of problems that can occur. Learn how to troubleshoot common H&E staining issues.
A first person view of how to manually stain slides using the H&E staining method.The protocol demonstrates use of Ehrlich's hematoxylin recipe which is desi...
For routine diagnosis, the use of H&E staining is by far preferred for viewing cellular & tissue structure detail. Learn about best practices, protocol & more.
H&E Staining Troubleshooting. The hematoxylin and eosin (H&E) stained tissue section is the cornerstone of anatomical pathology diagnosis. The H&E procedure stains the nucleus and cytoplasm contrasting colors to readily differentiate cellular components.
Review the basics of H&E staining and what optimal staining should look like. Identify common problems encountered with staining techniques. Consider solutions to common artifacts that influence stain results.