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H&E Troubleshooting Table . Page 1 of 7 . Problem Cause Solution . 1. Nuclei not crisp, “smudgy” nuclei, nuclear bubbling or no distinct chromatin pattern seen. There is no variation in the nuclear chromatin patterns among cells, and there is no variation in chromatin staining within one nucleus. a) Fixation is incomplete.
This document provides a troubleshooting table for problems that may occur with hematoxylin and eosin (H&E) staining of tissue samples. It lists three common problems - smudgy nuclei, lack of variation in nuclear staining, and poor contrast between nuclear and cytoplasmic staining.
a weak acid alcohol. Weak basophilic (nuclear) staining in an H&E stain is caused when the hematoxylin is too weak or wasn’t exposed to the specimen for adequate time. We recommend: Exposure time in hematoxylin is too limited: To solve exposure time issues, simply adjust the length of time the slide is in the hematoxylin stain solution
Though H&E stains are relatively simple to perform, there are a variety of problems that can occur. Learn how to troubleshoot common H&E staining issues.
H & E is the primary diagnostic technique for evaluation of morphology in the histopathology labs. One of the best nuclear stains. H & E provides easier identification of histological features than T-blue. It is easy and simple to use. Stains are inexpensive, yet reliable and informative. It is stable and durable stain, lasting years
Common problems, pitfalls and troubleshooting tips. H & E is the primary diagnostic technique for evaluation of morphology in the histopathology labs. One of the best nuclear stains. H & E provides easier identification of histological features than T-blue. It is easy and simple to use. Stains are inexpensive, yet reliable and informative. It ...
These general instructions apply to Hematoxylin and Eosin (H&E) Primary Staining reagents from Agilent. Always consult the specific Instructions for Use for your product for specific instructions on the use of the product. The information in this General Instructions for Primary Staining are guidelines only. A validated protocol is provided in ...
