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H&E Troubleshooting Table . Page 1 of 7 . Problem Cause Solution . 1. Nuclei not crisp, “smudgy” nuclei, nuclear bubbling or no distinct chromatin pattern seen. There is no variation in the nuclear chromatin patterns among cells, and there is no variation in chromatin staining within one nucleus. a) Fixation is incomplete.
For routine diagnosis, the use of H&E staining is by far preferred for viewing cellular & tissue structure detail. Learn about best practices, protocol & more.
Though H&E stains are relatively simple to perform, there are a variety of problems that can occur. Learn how to troubleshoot common H&E staining issues.
H&E Staining Troubleshooting. The hematoxylin and eosin (H&E) stained tissue section is the cornerstone of anatomical pathology diagnosis. The H&E procedure stains the nucleus and cytoplasm contrasting colors to readily differentiate cellular components.
Controlling the amount of hematoxylin staining is certainly key to achieving a good H&E stain, but what about eosin? In this first troubleshooting tutorial w...
This document provides a troubleshooting table for problems that may occur with hematoxylin and eosin (H&E) staining of tissue samples. It lists three common problems - smudgy nuclei, lack of variation in nuclear staining, and poor contrast between nuclear and cytoplasmic staining.
9 gru 2014 · H and E Part Two: Method, Tips and Troubleshooting. The complete method for H&E staining is contained in the tables below, but we’ll take a look at each of the stages in turn and explain the process which should help you should you need to troubleshoot. Dewax and rehydrate.
