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H&E Troubleshooting Table . Page 1 of 7 . Problem Cause Solution . 1. Nuclei not crisp, “smudgy” nuclei, nuclear bubbling or no distinct chromatin pattern seen. There is no variation in the nuclear chromatin patterns among cells, and there is no variation in chromatin staining within one nucleus. a) Fixation is incomplete.
a weak acid alcohol. Weak basophilic (nuclear) staining in an H&E stain is caused when the hematoxylin is too weak or wasn’t exposed to the specimen for adequate time. We recommend: Exposure time in hematoxylin is too limited: To solve exposure time issues, simply adjust the length of time the slide is in the hematoxylin stain solution
This document provides a troubleshooting table for problems that may occur with hematoxylin and eosin (H&E) staining of tissue samples. It lists three common problems - smudgy nuclei, lack of variation in nuclear staining, and poor contrast between nuclear and cytoplasmic staining.
Though H&E stains are relatively simple to perform, there are a variety of problems that can occur. Learn how to troubleshoot common H&E staining issues.
Staining problems Understanding the way the staining works is key to understanding and troubleshooting staining problems. It takes time effort on the techs part to learn how to correct staining errors.
Common problems, pitfalls and troubleshooting tips. H & E is the primary diagnostic technique for evaluation of morphology in the histopathology labs. One of the best nuclear stains. H & E provides easier identification of histological features than T-blue. It is easy and simple to use. Stains are inexpensive, yet reliable and informative. It ...
staining problems? Yes. Whether the problems are seen in hematoxylin, eosin, or any stain, wayward results can be categorized as: 1) too much stain, 2) too little stain, 3) wrong color, or 4) wrong site. See Tables 3 and 4. If the problem is too much stain, put less in by using a less concentrated stain for the same
