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H&E Troubleshooting Table . Page 1 of 7 . Problem Cause Solution . 1. Nuclei not crisp, “smudgy” nuclei, nuclear bubbling or no distinct chromatin pattern seen. There is no variation in the nuclear chromatin patterns among cells, and there is no variation in chromatin staining within one nucleus. a) Fixation is incomplete.
Though H&E stains are relatively simple to perform, there are a variety of problems that can occur. Learn how to troubleshoot common H&E staining issues.
a weak acid alcohol. Weak basophilic (nuclear) staining in an H&E stain is caused when the hematoxylin is too weak or wasn’t exposed to the specimen for adequate time. We recommend: Exposure time in hematoxylin is too limited: To solve exposure time issues, simply adjust the length of time the slide is in the hematoxylin stain solution
Protocol for H&E staining: While sections are in water, skim surface of hematoxalin with a Kimwipe to remove oxidized particles. Blot excess water from slide holder before going into hematoxalin. Coverslip slides using Permount (xylene based).
This document provides a troubleshooting table for problems that may occur with hematoxylin and eosin (H&E) staining of tissue samples. It lists three common problems - smudgy nuclei, lack of variation in nuclear staining, and poor contrast between nuclear and cytoplasmic staining.
General Instructions for H&E. For Primary Staining. Purpose. These general instructions apply to Hematoxylin and Eosin (H&E) Primary Staining reagents from Agilent. Always consult the specific Instructions for Use for your product for specific instructions on the use of the product.
Hematoxylin and eosin stain (H&E stain or HE stain) is a popular staining method in histology. The two stains were introduced in 1865 and 1875 by Bohmer and Fischer. It is the most widely used stain in medical diagnosis worldwide. When a pathologist/mohs surgeon looks at a biopsy or mohs section of a suspected cancer, the histological section ...
