Search results
H&E Troubleshooting Table . Page 1 of 7 . Problem Cause Solution . 1. Nuclei not crisp, “smudgy” nuclei, nuclear bubbling or no distinct chromatin pattern seen. There is no variation in the nuclear chromatin patterns among cells, and there is no variation in chromatin staining within one nucleus. a) Fixation is incomplete.
Though H&E stains are relatively simple to perform, there are a variety of problems that can occur. Learn how to troubleshoot common H&E staining issues.
For routine diagnosis, the use of H&E staining is by far preferred for viewing cellular & tissue structure detail. Learn about best practices, protocol & more.
This document provides a troubleshooting table for problems that may occur with hematoxylin and eosin (H&E) staining of tissue samples. It lists three common problems - smudgy nuclei, lack of variation in nuclear staining, and poor contrast between nuclear and cytoplasmic staining.
H&E Staining Troubleshooting. The hematoxylin and eosin (H&E) stained tissue section is the cornerstone of anatomical pathology diagnosis. The H&E procedure stains the nucleus and cytoplasm contrasting colors to readily differentiate cellular components.
Review the basics of H&E staining and what optimal staining should look like. Identify common problems encountered with staining techniques. Consider solutions to common artifacts that influence stain results.
The principle of the H&E staining procedure is the sequential application of two different stains. First is deparaffination in xylene or xylene substitute, Histo-Clear II is recommended, followed by hydration in declining concentration of ethanol ending up in water before application of the first stain hematoxylin.
