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Download scientific diagram | Examples of Hematoxylin and Eosin (H&E) stained histopathological images. (a) A sample image with nuclei that are well stained and have clear boundaries.
H&E staining. The most commonly used staining system is called H&E (Haemotoxylin and Eosin). H&E contains the two dyes haemotoxylin and eosin. Eosin is an acidic dye: it is negatively charged (general formula for acidic dyes is: Na + dye-). It stains basic (or acidophilic) structures red or pink. This is also sometimes termed 'eosinophilic'.
Hematoxylin stains DNA and RNA within cells. It also stains other negatively charged molecules. For example, the sulphated glycoproteins found in mucus and sulphated polysaccharides found in the extracellular matrix of cartilage.
Rough-endoplasmic reticulum - rER. The outer membrane of the rER is studded with ribosomes. This gives a basophilic staining to cytoplasm in H&E stains (due to the RNA content). The rER plays a major role in protein synthesis. It synthesises secretory proteins, and lysosomal enzymes.
Download scientific diagram | Hematoxylin and eosin (H&E) staining images of heart tissue samples from groups 1-4. H&E staining of (A) group 1, (B) group 2, (C) group 3 and (D)...
For routine diagnosis, the use of H&E staining is by far preferred for viewing cellular & tissue structure detail. Learn about best practices, protocol & more.
A basic dye used to stain the rough ER in neurones. Sudan Black and osmium. These dyes stain lipid-containing structures such as myelin a brownish-black colour.
