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Rough-endoplasmic reticulum - rER. The outer membrane of the rER is studded with ribosomes. This gives a basophilic staining to cytoplasm in H&E stains (due to the RNA content). The rER plays a major role in protein synthesis. It synthesises secretory proteins, and lysosomal enzymes.
- What is Histology: The Histology Guide
H&E staining. The most commonly used staining system is...
- What is Histology: The Histology Guide
H&E staining. The most commonly used staining system is called H&E (Haemotoxylin and Eosin). H&E contains the two dyes haemotoxylin and eosin. Eosin is an acidic dye: it is negatively charged (general formula for acidic dyes is: Na + dye-). It stains basic (or acidophilic) structures red or pink. This is also sometimes termed 'eosinophilic'.
Explain what color you expect the RER and the SER to stain under H&E. If there is a difference, why? Answer: RER should be basophilic because of the presence of ribosomes, whereas SER should appear more eosinophilic.
Endoplasmic reticulum (ER) is an internal membrane that forms branching networks of many interconnected sacs and tubes inside cells. Rough ER stays closer to the nucleus and coordinates protein synthesis. Smooth ER lacks ribosomes but specializes in lipid synthesis.
For routine diagnosis, the use of H&E staining is by far preferred for viewing cellular & tissue structure detail. Learn about best practices, protocol & more.
Within the cytoplasm the rough endoplasmic reticulum (ER) is readily seen in cells that are secreting large amount of protein. Because the rough ER contains a high concentration of ribosomes, it will stain blue in H&E samples and contrast nicely with the pinkish cytoplasm.
12 mar 2021 · That has been the case with hepatocytic inclusions detected in H&E-stained preparations, which appeared to correspond to secretory proteins that, due to genetic mutations, were retained within the rough endoplasmic reticulum (RER) and were deficient in plasma.
