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HEMATOXYLIN & EOSIN (H & E) STAIN PROTOCOL. PRINCIPLE: This protocol is applied in the routine staining of cationic and anionic tissue components in tissue sections. This is the standard reference stain used in the study of histochemical tissue pathology. SPECIMEN REQUIRED: Snap frozen human striated muscle. (Use the 2-methylbutane freezing method)
Protocol for H&E staining: While sections are in water, skim surface of hematoxalin with a Kimwipe to remove oxidized particles. Blot excess water from slide holder before going into hematoxalin. Coverslip slides using Permount (xylene based).
This procedure establishes a consistent process for preparing H&E slides from frozen or FFPE tissue samples using the automated H&E stainer and coverslip instruments. This
Hematoxylin and Eosin Stain For Fresh Frozen Section (H&E) PROCEDURE: 1. Take slides immediately from freezer and place in cold fixative (10% Neutral Buffered Formalin or 4% PFA) for 10 minutes 2. Rinse slides in 1X PBS, 2 times for 3 minutes each to remove OCT or other tissue embedding compound 3. Rinse in a gentle stream of tap water for 1 ...
This SOP provides a standardised method for performing Hematoxylin and Eosin (H&E) staining of paraffin-embedded tissue sections to ensure consistent and high-quality staining results. Scope. This SOP applies to all personnel in the histopathology laboratory involved in staining tissue sections using the H&E technique. Responsibilities.
To perform an H&E stain, follow the procedure below to re-hydrate, dehydrate and stain the tissue sections. Same solvents can be used for multiple tissue sections but should be changed daily or more often if they become contaminated (strongly colored by the stains). References: 1. Laboratory methods in histotechnology / edited by: Edna B ...
The principle of the H&E staining procedure is the sequential application of two different stains. First is deparaffination in xylene or xylene substitute, Histo-Clear II is recommended, followed by hydration in declining concentration of ethanol ending up in water before application of the first stain hematoxylin.
