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Though H&E stains are relatively simple to perform, there are a variety of problems that can occur. Learn how to troubleshoot common H&E staining issues.
H&E Troubleshooting Table . Page 1 of 7 . Problem Cause Solution . 1. Nuclei not crisp, “smudgy” nuclei, nuclear bubbling or no distinct chromatin pattern seen. There is no variation in the nuclear chromatin patterns among cells, and there is no variation in chromatin staining within one nucleus. a) Fixation is incomplete.
Though the H&E staining is a relatively simple method to perform, there are a variety of artifacts that can interfere with a good stain. Artifacts can be attributed to a variety of causes. 1. White spots in section after deparaffinization. 2. The nuclei too pale. 3. Nuclei overstained. 4. Red, reddish-brown nuclei. 5. Pale eosin staining. 6.
Review the basics of H&E staining and what optimal staining should look like. Identify common problems encountered with staining techniques. Consider solutions to common artifacts that influence stain results.
9 gru 2014 · The complete method for H&E staining is contained in the tables below, but we’ll take a look at each of the stages in turn and explain the process which should help you should you need to troubleshoot.
15 maj 2020 · There are numerous variables that can affect the quality of your H&E stain. In this post we will cover some common problems you may run into. The slide is too pink. If the slide is too pink it is generally a result of your eosin and can be solved by altering the time that you leave the slide in various steps within the H&E staining process.
28 sie 2018 · TROUBLESHOOTING IN H&E STAIN The hematoxylin is too dark (The nuclei are overstained), or diffuse hematoxylin staining of the cytoplasm The sections were stained too long in hematoxylin. Decolorize the section and restain, making appropriate adjustments in the staining time of hematoxylin.
