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To arrive at correct chromatic response, excess dye must be removed by treatment with dilute acid (differentiation). Gill No. 1 formulation is used as a progressive cytology stain, Gill formulations No. 2 and No. 3 may be used as progressive or regressive stains depending on length of staining time.
Gill's hematoxylin is an alum hematoxylin. It may be used as a progressive or regressive stain and is available in different concentrations. Because it is made with water and ethylene glycol, autoxidation of the stain is typically prevented over months, making it more stable than Harris hematoxylin.
20 sty 2024 · pH control. Dissolve ethylene glycol in distilled water. Add hematoxylin, then sodium iodate. Add aluminum sulfate and acetic acid. Stir for 1 hour at room temperature. Filter if necessary. Staining Characteristic.
Thermo Scientific™ Gill Hematoxylin 1, 2, and 3 are rapid progressive hematoxylins that are widely known and respected in the Histology and Cytology fields. They offer the convenience and choice of stain intensity.
Gill Hematoxylin solutions are nuclear stains intended for use in Histology and Cytology. Hematoxylin Solutions, Gill Nos. 1, 2 and 3 are for “In Vitro Diagnostic Use”. For professional use
Dako Gill’s 3 Hematoxylin is intended for primary staining of nuclei (blue) in formalin-fixed, paraffin-embedded tissue sections, frozen sections, and cell preparations. It is a ready-to-use reagent for use on Dako CoverStainer instrument.
PRINCIPLE AND RESULTS: This stain is intended for use by laboratory professionals to stain routinely prepared paraffin embedded tissue specimens (in vitro) to identify nuclei and cytoplasm. Nuclei are regressively stained blue to purple and cytoplasm, collagen, and muscle pink to red.
