Search results
20 maj 2023 · We introduce an atomistic and machine-learning-based approach, called high-throughput Functional Libraries (htFuncLib), that designs a sequence space in which mutations form low-energy...
- A novel protocol to detect green fluorescent protein in unfixed, snap ...
The green fluorescent protein (GFP) is a powerful reporter...
- A novel protocol to detect green fluorescent protein in unfixed, snap ...
4 wrz 2020 · The green fluorescent protein (GFP) is a powerful reporter protein that allows labeling of specific proteins or entire cells. However, as GFP is a small soluble protein, it easily crosses ...
23 sie 2024 · Method. In order to generate a GFP fusion protein, a cDNA that encodes the protein of interest must be available or obtainable. Then, through molecular cloning, the cDNA for GFP must be cloned upstream or downstream of the cDNA for the protein of interest, in frame, creating a fusion protein. Next, the modified DNA must be introduced into the ...
11 maj 2016 · Here we visualize an extensive region of the local fitness landscape of the green fluorescent protein from Aequorea victoria (avGFP) by measuring the native function (fluorescence) of tens of...
1 mar 2022 · Green fluorescent protein (GFP), a fluorescent marker extracted from Aequorea victoria, has been a prominent tool for protein visualisation in modern biomedical research. When properly folded, it emits green fluorescence upon UV illumination.
The green fluorescent protein (GFP) and a variety of GFP-like homologues that colour tissues of many reef organisms have revolutionized biological and biomedical research by providing the means to fluorescently tag and visualize the activity of genes and proteins in living cells. This chapter describes how we can further capitalize on what ...
The GFP polyclonal and monoclonal antibodies can be used to confirm GFP protein expression by Western blots and to correlate levels of GFP protein expression with fluorescence intensity (detected with microscopy or flow cytometry).
