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18 gru 1998 · The use of green fluorescent protein (GFP) as a reporter for protein localization in Escherichia coli was explored by creating gene fusions between malE (encoding maltose-binding protein [MBP]) and a variant of gfp optimized for fluorescence in bacteria (GFPuv).
- Green fluorescent protein as a reporter for macromolecular localization ...
Localization of GFP fusion proteins can be analyzed in live...
- Green fluorescent protein as a reporter for macromolecular localization ...
16 paź 2001 · Use of the green fluorescent protein (GFP) of Aequorea victoria as a reporter for protein and DNA localization has provided sensitive, new approaches for studying the organization of the bacterial cell, leading to new insights into diverse cellular processes.
26 kwi 2023 · Here the authors exploit this data to offer an unbiased overview of complex sequence-structure-function relationships in the protein universe.
19 lip 2011 · The aim of these exercises is to teach the students how to manipulate three-dimensional protein structural models and to analyze, using site-directed mutagenesis, key residues in the protein structure. The green fluorescent protein (GFP)1 from the jellyfish Aequorea victoria is ideal for this purpose, for
3 maj 2016 · Using a single domain antibody (also called nanobody) with high affinity for green fluorescent protein (GFP), we constructed a system that allows for fast, fluorescence-based detection of displayed proteins.
3 sie 2018 · GFPs and their variants, due to their intrinsic fluorescence properties, are extensively being used nowadays in cell-based assays. This review focuses on structure and function of GFP and its derivatives, mechanism emission and their use in the development of FRET-based sensors for metabolites.
Localization of GFP fusion proteins can be analyzed in live bacteria, therefore eliminating potential fixation artifacts and enabling real-time monitoring of dynamics in situ. Such real-time studies have been facilitated by brighter, more soluble GFP variants.
