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18 gru 1998 · The use of green fluorescent protein (GFP) as a reporter for protein localization in Escherichia coli was explored by creating gene fusions between malE (encoding maltose-binding protein [MBP]) and a variant of gfp optimized for fluorescence in bacteria (GFPuv).
16 paź 2001 · Two strategies to investigate chromosome behavior in bacteria include the use of GFP-fused partitioning proteins that bind specifically to the origin of replication (ori), and by using a GFP–LacI reporter/repressor hybrid protein.
3 maj 2016 · Using a single domain antibody (also called nanobody) with high affinity for green fluorescent protein (GFP), we constructed a system that allows for fast, fluorescence-based detection of displayed proteins.
4 wrz 2020 · The green fluorescent protein (GFP) is a powerful reporter protein that allows labeling of specific proteins or entire cells. However, as GFP is a small soluble protein, it easily crosses...
Green fluorescent protein (GFP)-like chromophores strictly requires molecular oxygen for maturation of fluorescence, which restrict the study of microorganisms in low-oxygen environments. In this work, we have developed a noninvasive cyan-green fluorescent based reporter system for real-time tracking of LAB that is functional under anoxic ...
8 kwi 2024 · Introduction. Fluorescent proteins (FPs) have become indispensable tools for a broad spectrum of microbiological research and applications. The diverse repertoire of FPs enables in vivo monitoring of gene expression or protein-localization from bacterial populations to single cells.
3 kwi 2001 · Go to: Abstract. We have formulated a numerical model that simulates the accumulation of green fluorescent protein (GFP) in bacterial cells from a generic promoter- gfp fusion.
