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Synergisms between the suitability of GFP-like proteins as precisely targeted intracellular genetic tags, the rapid development of imaging techniques, and data analysis have boosted the use of GFP in the biological sciences.
The workflow below describes two examples of how WesTM works to streamline your bioprocess engineering workflows with GFP, including screening for residual GFP following co-transfection of host cells, and monitoring expression of GFP-tagged proteins in HEK 293 cells.
INSTRUCTIONS . Aim: To understand how to analyze DNA and protein sequences using the bioinformatics tool BLAST (Basic Local Alignment Search Tool). These analyses will help you better understand the origin and diversity of fluorescent proteins used in biological research. PART I: Aligning DNA Sequences . 1.
Introduction. This application note describes how the green fluorescent protein (GFP) expressed from Bio-Rad’s pGLO plasmid can be used to help illustrate and teach the central dogma of biology, from the transformation of DNA to the expression of a protein to the visualization of a trait.
There are several mutant GFP genes that encode mutant proteins with small differences in their nucleotide and amino acid sequences. This bioinformatics activity was designed for an upper level course in molecular biology.
1 mar 2022 · Green fluorescent protein (GFP), a fluorescent marker extracted from Aequorea victoria, has been a prominent tool for protein visualisation in modern biomedical research. When properly folded, it emits green fluorescence upon UV illumination.
The purified protein is then identified using polyacrylamide gel electrophoresis (PAGE). This lab is divided into two parts: Purification of GFP by HIC and PAGE Analysis of Purified GFP. Part A provides a procedure for purifying GFP.
