Search results
Synergisms between the suitability of GFP-like proteins as precisely targeted intracellular genetic tags, the rapid development of imaging techniques, and data analysis have boosted the use of GFP in the biological sciences.
23 sie 2024 · Method. In order to generate a GFP fusion protein, a cDNA that encodes the protein of interest must be available or obtainable. Then, through molecular cloning, the cDNA for GFP must be cloned upstream or downstream of the cDNA for the protein of interest, in frame, creating a fusion protein. Next, the modified DNA must be introduced into the ...
There are several mutant GFP genes that encode mutant proteins with small differences in their nucleotide and amino acid sequences. This bioinformatics activity was designed for an upper level course in molecular biology.
The cloning and sequencing of the GFP gene from A. victoria (2, 6) has permitted expression of this protein in heterologous systems. The GFP expressed in both prokaryotic and eukaryotic cells yields green fluorescence when excited by blue or UV light.
GFP has become well established as a marker of gene expression and protein targeting in intact cells and organisms. Mutagenesis and engineering of GFP into chimeric proteins are opening new vistas in physiological indicators, biosensors, and photochemical memories. CONTENTS.
15 sty 2016 · Green fluorescent protein (GFP) from the jellyfish Aequorea victoria and its homologs from diverse marine animals are widely used as universal genetically encoded fluorescent labels.
15 lip 2019 · Superfolder variants of (top) green fluorescent protein (GFP) and (bottom) DsRed. The β-can structures of GFP (PDB code 1ema) and DsRed (PDB code 1g7k) share ~23% sequence identity.
