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31 paź 2018 · Following Ficoll purification, a portion of each marrow aspirate was frozen and stored at −120 °C to measure the effects of cryogenic storage on cell cycle distribution in human bone marrow cell populations.
1 maj 2018 · Using an example of spinach leaves with initial osmotic pressure of ∼ 0.3 Osm, equilibrium freezing to −4.5 °C should result in cell sap concentration of ∼2.4 Osm (efflux of ∼88% of freezable water). Why is injury substantially greater after 5.5 h versus 3 h of freezing despite equivalent freeze-desiccation?
1 lut 2021 · However, the inevitable water-to-ice phase transition (intracellular and extracellular ice) at the freezing temperature usually leads to cells sustaining mechanical ice damage. The elevated solute concentration due to ice formation also results in irreversible injury to cells after undergoing freeze–thaw cycles.
4 mar 2019 · The cells were frozen down using four cooling rates 0.1 °C min −1 (using a VIA freeze controlled rate freezer), 1 °C min −1, 10 °C min −1 (using a Planer controlled rate freezer) and ~159 ...
16 lis 2021 · Intracellular glass transition temperature for a range of cell types, determined by DSC analysis. The temperature providing optimal cell recovery following cryopreservation of the nucleate, mammalian cell lines is presented as the safe temperature for transfer to long term storage.
2 lis 2017 · Survival of cells from the rigors of freezing and thawing in cryopreservation procedures is only attained by using appropriate cryoprotective agents, and in general, these techniques are applicable to isolated cells in suspension or small aggregates of cells in simple tissues.
1 gru 2010 · The equilibrium freezing temperature of −8 °C for the 4 M sorbitol solution was determined from the melting point of the solution measured using FTIR spectroscopy during a freeze/thaw cycle. After exposure to hyperosmotic stress, HFFs were returned back to the isotonic solution for subsequent analysis ( Fig. 1 a).
