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The process of freezing cells or tissues and depositing them in liquid nitrogen at –196 °C is called cryopreservation. Sub-zero temperature is not a physiological condition for cells and water ice crystals represent the main problem since they ...
1 lut 2022 · The phase change of water occurs in biological samples during freezing and introduces significant changes to the processed materials. The phase change phenomenon includes complex processes at the macro and micro levels.
18 gru 2014 · Pure water will freeze at approximately -39°C in the absence of nucleation sites. In practice, though, homogenous nucleation is not often seen because of the rarity of completely pure water.
31 paź 2018 · Following Ficoll purification, a portion of each marrow aspirate was frozen and stored at −120 °C to measure the effects of cryogenic storage on cell cycle distribution in human bone marrow cell populations.
1 maj 2018 · Since ice has lower vapor pressure at a given subfreezing temperature than that of pure liquid water [41], osmotically active water is drawn from the protoplasm and cell walls and freezes extracellularly causing freeze-desiccation and ‘caving-in’ of cells, termed ‘cytorrhysis’.
1 sty 2021 · This data provides a fascinating insight into the crystal structure dependent behaviour during phase change of frozen cell therapies and its effect on live cell suspensions.
Cells can endure storage at low temperatures such as--196 degrees C for centuries. The challenge is to determine how they can survive both the cooling to such temperatures and the subsequent return to physiological conditions. A major factor is whether they freeze intracellularly.
