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8 lut 2017 · Mitotic spindle assembly results in an antiparallel, bipolar microtubule array that consists of three broad categories of microtubules (MTs): kinetochore MTs (K-MTs), astral MTs (A-MTs) and non...
7 wrz 2000 · The mitotic spindle is a bipolar, self-organizing microtubule (MT)-based machine that uses energy liberated from nucleotide hydrolysis to segregate sister chromatids accurately into the...
15 cze 2012 · The current view is that MTs that originate from the different pathways cooperate to form the spindle: the centrosome asters define the position of the two spindle poles and the axis of cell division; the chromosomal MTs facilitate the capture of the kinetochores and the formation of the K-fibers; and MT amplification by the Augmin complex ...
4 dni temu · The tail contains multiple phosphorylation sites 11, 66 (Figure 1D). The BimC box 6 contains a conserved Cdk1 site, and alanine mutants of the consensus site threonine cause a loss of metazoan kinesin-5 spindle localization. 7, 40, 42, 43 Antibodies to the phosphorylated Drosophila kinesin-5 Cdk1 site show enhanced localization in the spindle midzone. 55 The human kinesin-5/Eg5 tail contains a ...
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1 lis 2020 · The cortical dynein captures MTs and generates a pulling force, which is essential for the positioning of the spindle within the cell (see Fig. 2 C). In vitro experiments have shown that dynein can maintain the connection to a shrinking MT for several seconds against pulling forces up to 5 pN [65].
