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1. www.sciencedirect.com › science › articleDevelopment of an analytical method for chlorophyll pigments...

   www.sciencedirect.com › science › article

   8 lut 2020 · Reversed-phase supercritical fluid chromatography (RP-SFC), which can be achieved with a non-polar C18-bonded phase, is an interesting alternative for the separation of this type of non-polar compounds. With regards to the organic solvents used in NARP-LC, carbon dioxide has many advantages: ecological, economic and physico-chemical.

2. www.chem.ucla.edu › ~bacher › CHEM14CLIsolation of Chlorophyll and Carotenoid Pigments from Spinach

   www.chem.ucla.edu › ~bacher › CHEM14CL

   The major advantages of column chromatography are its ability to handle larger amounts of material (compared to GC, TLC and HPLC) and the ability to change the eluting solvent throughout the course of the separation. This allows one to remove one component while a desired product remains essentially unmoved.

3. link.springer.com › chapter › 10Analytical Protocols in Chlorophyll Analysis | SpringerLink

   link.springer.com › chapter › 10

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   9 sie 2020 · A deeper analysis has been achieved through the mass spectrophotometric (MS) studies of chlorophyll compounds of main microalgae and cyanobacteria, including the analysis of the fragmentation pathways to increase consistency of the identification.

4. prometheusprotocols.net › determination-of-chlorophylls-and-carotenoids-by-hplcDetermination of chlorophylls and carotenoids by HPLC

   prometheusprotocols.net › determination-of-chlorophylls-and-carotenoids-by-hplc

   The solvents must be HPLC grade or vacuum filtered through a nylon membrane of 0.2 μm pore size before use (as for the Tris buffer in solvent A) in order to avoid problems in the accuracy of the system.

5. link.springer.com › chapter › 10Plant Photosynthetic Pigments: Methods and Tricks for Correct ...

   link.springer.com › chapter › 10

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   18 sie 2018 · High performance liquid chromatography (HPLC) is one of the best options to characterise the exact pigment composition in a plant tissue, and it is the standard method to quantify individual carotenoids.

6. link.springer.com › article › 10Evaluation of the extraction and stability of chlorophyll-rich...

   link.springer.com › article › 10

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   24 sie 2020 · PLE studies have reported the effect of extraction temperature or a comparison of PLE to maceration (MAC) (typically 6 h at room temperature), Soxhlet extraction (2 h) and ultrasound-assisted extraction (UAE) (2 h), for instance, with a solvent mixture of ethanol and water 90/10 (v/v) [22, 23].

7. www.sciencedirect.com › science › articleExtraction, Separation, Estimation, and Isolation of the...

   www.sciencedirect.com › science › article

   Fresh plant material must be extracted with solvents that remove most of the water before the pigments can be dissolved. Unless an excess of solvent is employed for the extraction, the chlorophylls may be oxidized or hydrolyzed (or both), yielding a variety of the products indicated in Sections V, β and C (73, 76).