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1. www.sciencedirect.com › science › articleDevelopment of an analytical method for chlorophyll pigments...

   www.sciencedirect.com › science › article

   8 lut 2020 · With regards to the organic solvents used in NARP-LC, carbon dioxide has many advantages: ecological, economic and physico-chemical. Moreover, it can be mixed with many other solvent (often called modifier) to tune the polarity of the mobile phase, inducing large changes in retention and sometimes great improvement in the separation performances.

2. www.sciencedirect.com › science › articleExtraction, Separation, Estimation, and Isolation of the...

   www.sciencedirect.com › science › article

   The chlorophylls are altered rapidly upon exposure to visible light. This reaction varies with the solvent. It occurs when the pigments are on chromatographic columns. For example, a zone of Chl a formed in a column of powdered sugar with benzene as the solvent and then exposed to sunlight soon yields a gray region next to the glass. When the ...

3. prometheusprotocols.net › determination-of-chlorophylls-and-carotenoids-by-hplcDetermination of chlorophylls and carotenoids by HPLC

   prometheusprotocols.net › determination-of-chlorophylls-and-carotenoids-by-hplc

   The solvents must be HPLC grade or vacuum filtered through a nylon membrane of 0.2 μm pore size before use (as for the Tris buffer in solvent A) in order to avoid problems in the accuracy of the system.

4. link.springer.com › chapter › 10Analytical Protocols in Chlorophyll Analysis | SpringerLink

   link.springer.com › chapter › 10

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   9 sie 2020 · A deeper analysis has been achieved through the mass spectrophotometric (MS) studies of chlorophyll compounds of main microalgae and cyanobacteria, including the analysis of the fragmentation pathways to increase consistency of the identification.

5. link.springer.com › article › 10A rapid method for developing chlorophyll equations using...

   link.springer.com › article › 10

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   14 paź 2024 · In the present study, the aim was to demonstrate how to calculate chlorophyll formulae for a new solvent using the readily available equations for Chl a, a + b, a + c 2, a + c 1 c 2 and d + a based on a benchmark solvent.

6. link.springer.com › article › 10Evaluation of the extraction and stability of chlorophyll-rich...

   link.springer.com › article › 10

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   24 sie 2020 · In this study, a rapid (less than 10 min) analytical method by reverse-phase supercritical fluid chromatography was developed with an isocratic mobile phase, enabling the separation of 11 compounds, chlorophyll a and b, pheophytin a and numerous allomers or epimers.

7. plantmethods.biomedcentral.com › articles › 10Simple extraction methods that prevent the artifactual conversion...

   plantmethods.biomedcentral.com › articles › 10

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   19 cze 2013 · Using DMF as an extractant is simple and suitable with Arabidopsis samples. However, this solvent cannot completely block the formation of chlorophyllide in thicker leaves.