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1. www.sciencedirect.com › science › articleDevelopment of an analytical method for chlorophyll pigments...

   www.sciencedirect.com › science › article

   8 lut 2020 · Reversed-phase supercritical fluid chromatography (RP-SFC), which can be achieved with a non-polar C18-bonded phase, is an interesting alternative for the separation of this type of non-polar compounds. With regards to the organic solvents used in NARP-LC, carbon dioxide has many advantages: ecological, economic and physico-chemical.

2. prometheusprotocols.net › function › tissue-chemistryChlorophyll extraction and determination – PROMETHEUS

   prometheusprotocols.net › function › tissue-chemistry

   Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy.

3. www.purdue.edu › science › science-expressANALYSIS OF PLANT PIGMENTS USING PAPER CHROMATOGRAPHY - Purdue...

   www.purdue.edu › science › science-express

   Chlorophyll a is slightly soluble in a 3:1:1 mixture of petroleum ether, acetone, and water. Carotenoids are very soluble in this solvent system. These solubility differences will allow the separation of chlorophyll a from the carotenoids and chlorophyll b on a paper chromatogram.

4. prometheusprotocols.net › determination-of-chlorophylls-and-carotenoids-by-hplcDetermination of chlorophylls and carotenoids by HPLC

   prometheusprotocols.net › determination-of-chlorophylls-and-carotenoids-by-hplc

   The solvents must be HPLC grade or vacuum filtered through a nylon membrane of 0.2 μm pore size before use (as for the Tris buffer in solvent A) in order to avoid problems in the accuracy of the system.

5. www.tutorchase.com › biology › 13/1/6-pigment-separation-by-chromatography13.1.6 Pigment Separation by Chromatography - TutorChase

   www.tutorchase.com › biology › 13/1/6-pigment-separation-by-chromatography

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   Using a non-polar solvent in the chromatography of chloroplast pigments is critical because the pigments themselves are non-polar compounds. Non-polar solvents effectively dissolve these pigments, allowing them to travel up the chromatography paper or TLC plate.

6. link.springer.com › chapter › 10Analytical Protocols in Chlorophyll Analysis | SpringerLink

   link.springer.com › chapter › 10

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   9 sie 2020 · A deeper analysis has been achieved through the mass spectrophotometric (MS) studies of chlorophyll compounds of main microalgae and cyanobacteria, including the analysis of the fragmentation pathways to increase consistency of the identification.

7. link.springer.com › article › 10Evaluation of the extraction and stability of chlorophyll-rich...

   link.springer.com › article › 10

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   24 sie 2020 · In this study, a rapid (less than 10 min) analytical method by reverse-phase supercritical fluid chromatography was developed with an isocratic mobile phase, enabling the separation of 11 compounds, chlorophyll a and b, pheophytin a and numerous allomers or epimers.