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Automated DNA Sequencing. Automated sequencing has been developed to sequence a really large amount of DNA. This procedure uses the principle of the Sanger chain-termination method.
The sequencing method developed by Fred Sanger forms the basis of automated cycle sequencing reactions today. Fluorescent dyes are added to the reactions, and a laser within an automated DNA sequencing machine is used to analyze the DNA fragments produced.
Each reaction involves a single-stranded template, a specific primer to start the reaction, the four standard deoxynucleotides (dATP, dGTP, dCTP, and dTTP), and DNA polymerase.
Automated DNA Sequencing Chemistry Guide. This updated guide provides the following: ♦ An introduction to automated DNA sequencing ♦ Descriptions of Applied Biosystems sequencing instruments, chemistries, and software ♦ Detailed protocols for preparing DNA templates, performing cycle sequencing,
the DNA sequencing reactions are combined and co-electrophoresed, and the DNA fragments generated in each reaction are detected near the bottom of the gel during the electrophoresis and identified by their color. Thus, the DNA sequence is encoded in the order in
Automated DNA sequencing involves cycle sequencing using a DNA thermal cycler which incorporates four different fluorescent dye labels (for A, C, T, and G) into DNA extension products using either 5′-dye labeled primers (dye primers) or 3′-dye labeled dideoxynucleotide triphosphates (dye terminators).
Go to: Why Sequence the Entire Human Genome? There is general agreement in the biological sciences community that a physical map of the human genome, as represented in a set of cloned overlapping DNA fragments, is a worthwhile goal.
