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  1. In this review, we selected important tools that enable access to homogeneously phosphorylated protein and discuss examples that demonstrate how they can be applied. Firstly, we discuss the synthesis of phosphopeptides and proteins through chemical and enzymatic means and their advantages and limitations.

  2. 8 cze 2014 · For the majority of each simulation, the phosphate tail of the ATP remained in contact with basic residues Lys12, Arg15 and Lys20 on the N-terminal α-helix, thus demonstrating the importance...

  3. 15 lut 2023 · The PEP/PK system for generation of ATP from ADP is generally superior to the AcK/acetyl phosphate system because PEP is more stable than acetyl phosphate. Additional advantages of PK include its commercial availability, lower sensitivity to oxygen, and greater stability when immobilized.

  4. 4 paź 2012 · In this review, we focus on P2X receptor mediated ATP signaling in the brain, discussing general themes pertinent to mechanisms and neuromodulation at the molecular, cellular, systems and disease levels.

  5. 1 sty 1996 · In regard to energy transfer, the critical part of the ATP molecule is its phosphate tail, and, in particular, its two terminal phosphate groups (P and y phosphates). Each is linked to the neighboring phosphate by an acid anhydride link.

  6. 22 lut 2021 · Examination of this set reveals β–γ-coordinated (C2) structures with extended phosphate tail configurations, with a mean value of 161° for the α–β–γ-phosphate angle.

  7. 20 lis 2018 · This review provides a history of observing actin filaments under light microscopy, an overview of dynamic properties of ATP hydrolysis at the end of actin filament, and structural views of γ-phosphate release.

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