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1 lis 2015 · The Effects of Arabinose Concentration on GFP Expression. By Emily Sidansky. Abstract: In this project, the central dogma was demonstrated, showing replication, transcription, reverse....
To detect the effect of arabinose-independent araE expression on population homogeneity and cell-specific expression, the gfpuv gene was placed under control of the arabinose-inducible araBAD promoter (PBAD) on the pMB1-derived plasmid pBAD24.
Green fluorescent protein (GFP) has gained widespread use as a tool to visualize spatial and temporal patterns of gene expression in vivo. However, it is not generally accepted that GFP can also be used as a quantitative reporter of gene expression.
The AraC protein will bind to the arabinose, allowing GFP transcription to mRNA to take place. This mRNA is then translated into green fluorescent protein (GFP) , a process known as gene expression. As the cells produce GFP, they fluoresce a bright green.
1 lut 2021 · YfbF-GFP, a possible inner MP with two transmembrane domains [66], showed the highest expression in both strains with 100 mM l -arabinose + 1 mM IPTG, indicating that overproduction of this specific protein is not limited by too high transcript levels of the GOI (Fig. 8).
3 paź 2023 · Our data demonstrate that the addition of 0.005% arabinose is sufficient to maximally induce P BAD-controlled expression of plasmid-encoded superfolder green fluorescent protein (sfGFP) (Fig....
26 lut 2024 · Systematic benchmarking of mBaoJin against popular green fluorescent proteins and other recently introduced monomeric and pseudomonomeric derivatives of StayGold established mBaoJin as a bright...
