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1 lis 2015 · To find whether the hypothesis was supported or failed to be supported, the experiment analyzed differential expression of GFP from a transformation of MM294 E. coli with pGLO plasmid and used...
3 paź 2023 · To facilitate new approaches for research and biotechnological applications, we here develop a set of arabinose-inducible artificial transcription factors (ATFs) using CRISPR/dCas9 and...
1 lut 2021 · YfbF-GFP, a possible inner MP with two transmembrane domains [66], showed the highest expression in both strains with 100 mM l -arabinose + 1 mM IPTG, indicating that overproduction of this specific protein is not limited by too high transcript levels of the GOI (Fig. 8).
After only 2 h of induction with arabinose, the cultures produced enough green fluorescent protein (GFP) to give a quantifiable fluorescence signal. Prior to arabinose addition (time zero), both cultures displayed background fluorescence intensities.
In both cases, a tightly regulated expression system is critical for maximizing recombinant protein yields. Based on the araBAD operon, which controls the arabinose metabolic pathway in E. coli (1,2,3), the pBAD Expression System allows you to precisely modulate lev-els to optimize yields.
Addition of arabinose sugar to the growth media will cause RNA polymerase to start transcribing the GFP gene (i.e. making mRNA molecules). The cellular machinery (e.g. ribosomes) will translate this mRNA into corresponding GFP protein.
The pBAD expression system allows tightly controlled, titratable expression of your protein through the regulation of specific carbon sources such as glucose, glycerol, and arabinose. pBAD is ideal for expressing toxic proteins and optimizing protein solubility in E. coli.
