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1 lis 2015 · The Effects of Arabinose Concentration on GFP Expression. By Emily Sidansky. Abstract: In this project, the central dogma was demonstrated, showing replication, transcription, reverse....
3 paź 2023 · To facilitate new approaches for research and biotechnological applications, we here develop a set of arabinose-inducible artificial transcription factors (ATFs) using CRISPR/dCas9 and...
26 lut 2024 · The high brightness and photostability of the green fluorescent protein StayGold make it a particularly attractive probe for long-term live-cell imaging; however, its dimeric nature precludes...
After only 2 h of induction with arabinose, the cultures produced enough green fluorescent protein (GFP) to give a quantifiable fluorescence signal. Prior to arabinose addition (time zero), both cultures displayed background fluorescence intensities.
21 gru 2016 · During the absence of arabinose, AraC acts on araBAD as repressor and as activator in the presence of arabinose (Schleif 2010). It is also dependent on the activation by cAMP receptor protein, which renders the system prone to catabolite repression.
1 lut 2021 · YfbF-GFP, a possible inner MP with two transmembrane domains [66], showed the highest expression in both strains with 100 mM l -arabinose + 1 mM IPTG, indicating that overproduction of this specific protein is not limited by too high transcript levels of the GOI (Fig. 8).
Arabinose is a five-carbon sugar that is found widely in nature and can serve as a sole carbon source in many bacteria. The protein products from three genes (araB, araA, and araD) are needed for arabinose degradation in members of the Enterobacteriaceae family, such as E. coli and S. typhimurium, and these genes form a cluster abbreviated araBAD.
