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CRISPR 101. January 2021 | Third Edition. About. This eBook covers practical experimental advice such as tips for planning your CRISPR experiment and designing your gRNA and how to use pooled libraries in genome-wide screens.
This guide will provide a basic understanding of CRISPR biology, introduce the various applications of CRISPR, and help you get started using CRISPR in your own research.
Addgene is working with the leading scientists in the field to assemble the reagents and information you need to use CRISPR technology in your own lab. Browse plasmids below or check out our CRISPR resources on how to start using CRISPR in your lab.
Download a PDF of CRISPR Cheat Sheet here! Sequences of DNA found in bacteria that allow the bacteria to target and destroy viruses that infect them. Commonly referred to as a bacterial immune system. Modifying the DNA sequence of an organism’s genome usually for a research or applied goal.
Applications of CRISPR. Cut: CRISPR can be used to cut the DNA sequence. With a repair template, CRISPR can be used to introduce a functional change in the DNA sequence. Base Editors: Modified versions of CRISPR . the DNA sequence without completely cutting the DNA. RNA Editors: CRISPR nucleases that cut or . modify RNA as opposed to DNA ...
lentiviral CRISPR plasmids targeting a single genomic locus. lentiCRISPRv2 (one vector system): This plasmid contains two expression cassettes, hSpCas9 and the chimeric guide RNA. The vector can be digested using BsmBI, and a pair of annealed oligos can be cloned into the single guide RNA scaffold.
Browse Addgene’s curated list of CRISPR resources and references, including gRNA design tools, CRISPR FAQs, and community forums.
