Search results
1 sty 2024 · V (D)J recombination is tightly regulated during lymphocyte development. RAG proteins initiate the reaction from their location in recombination centres in various antigen receptor loci. Chromatin loop extrusion then presents RAG with chromatin containing substrate segments embedded within megabase DNA segments.
V (D)J recombination (variable–diversity–joining rearrangement) is the mechanism of somatic recombination that occurs only in developing lymphocytes during the early stages of T and B cell maturation. It results in the highly diverse repertoire of antibodies/immunoglobulins and T cell receptors (TCRs) found in B cells and T cells, respectively.
19 kwi 2002 · V(D)J recombination is initiated via introduction of DNA double-strand breaks (DSBs) between the V, D, and J segments and flanking RSs; subsequently, RS ends are precisely joined, while coding ends are modified via a process that involves potential nucleotide loss and potential nucleotide addition.
V (D)J recombination assembles immunoglobulin and T cell receptor genes during lymphocyte development through a series of carefully orchestrated DNA breakage and rejoining events. DNA cleavage requires a series of protein-DNA complexes containing the RAG1 and RAG2 proteins and recombination signals that flank the recombining gene segments.
V(D)J recombination is of fundamental importance to the generation of diverse antigen receptor repertoires. We review our current understanding of the V(D)J re-combination reaction and how it is regulated during lymphocyte development. We also discuss how de-fects in the mechanism or regulation of V(D)J recombi- nation can lead to human disease.
1 sty 2015 · During lymphocyte development, the diverse repertoire of functional antigen receptors is created by the process of V(D)J recombination, in which various segments of an antigen receptor locus are brought together by several highly coordinated DNA cleavage and repair steps.
V(D)J recombination undergoes regulation at multiple levels, including the regulation of RAG1/2 activity, subnuclear reposition, spatial conformation of chromatin, and Vκ recombination, and has some specific regulation mechanisms.
